Detecting single base substitutions, mismatches and bulges in DNA by temperature gradient gel electrophoresis and related methods.
نویسندگان
چکیده
Temperature gradient gel electrophoresis (TGGE) and related methods can separate DNA fragments that differ by a single base pair or defect. This article describes the basic features of TGGE, and reviews the theoretical model of DNA unwinding and its ability to predict DNA mobility in a temperature gradient gel. Recent applications of TGGE and related methods that were directed at detecting point mutations, and evaluating the effects of single site defects are also reported.
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ورودعنوان ژورنال:
- Journal of chromatography. A
دوره 806 1 شماره
صفحات -
تاریخ انتشار 1998